Seed production and culture of Mystus guliolocally known as Nuna Tengra can provide an assured livelihood option to small and marginal farmers of the Sundarban in West Bengal. Mystus gulio is a commercially important brackishwater catfish, which is an important small indigenous fish species (SIS) of the Sundarban delta. It can tolerate a wide range of salinity and has high market demand with price ranging Rs. 200- 500kg. Due to overexploitation and environmental degradation, availability of seeds from nature has been decreased. In order to conserve the species, and promote scientific farming and diversified aquaculture, Kakdwip Research Centre (KRC) of ICAR-Central Institute of Brackishwater Aquaculture (CIBA) has developed a complete package comprising of homestead/ backyard hatchery technique and grow-out culture of this fish in brackishwater system. Breeding technology package of Mystus gulio in backyard hatchery system comprises of broodstock development, maturity assessment, induction of spawning, incubation and hatching of eggs, and larval rearing in brackishwater environment. Total cost of production of a 30-day old seed is only Re. 0.30 and it has a market selling price of Re.1, which is economically lucrative. Cost effective backyard hatchery operation requires less capital and less labour. The woman member of a family can easily manage these less cumbersome and easy activities. A small farmer with a minimum operational cost of Rs 20000 can easily earn Rs 42000 in a breeding season of six months. Many rural people, small and marginal farmers may get employment through production and supply of seeds.